Figure 2

Tetramer assay specificity. (A) TIL cell binding: % tetramer positive cells are shown based on data in the upper right quadrant from each of the 4 blots. TIL1520 (top panel) were stained with negative tetramer (left) and gp100 tetramer (right). TIL1143 (bottom panel) were stained with negative tetramer (left) and MART-1 tetramer (right). (B) MART-1 Jurkat cell binding: % tetramer positive cells are shown based on data in the upper right quadrant from MART-1 Jurkat cell blots (lower panel) stained with irrelevant gp100 tetramer (left) or relevant MART-1 tetramer (right). Control Jukat cells (upper panel) were stained with both tetramers (% tetramer positive cells are <0.05%, data not shown).