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Figure 1 | Journal of Translational Medicine

Figure 1

From: Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

Figure 1

Detection of tetramer positive cells among PBMC. Gating sequence is shown in the upper panel. (A) R1-Lymphocyte gate, FSC (x-axis) vs. SSC (y-axis). (B) R2-Viable cell gate, FSC (x-axis) vs. PI (y-axis). (C) R3-CD8+ cell gate, FSC (x-axis) vs. CD8 FITC (y-axis). Flu-tetramer positive cells are shown in (D) Flu tetramer positive cells, CD8 FITC (x-axis) vs. Flu tetramer PE (y-axis), gated on R1 and R2 for viable lymphocyte. CD8 negative cells are shown (with R3 off), demonstrating assay specificity.

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