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Table 2 Peptide and tumor reactivity of gp100154–162 specific CD8+ T cell clones *

From: Use of high throughput qPCR screening to rapidly clone low frequency tumour specific T-cells from peripheral blood for adoptive immunotherapy

 

Peptide Reactivity

 

Tumor Reactivity

gp100154 (M)

HIV (M)

A2+/gp100+

A2+/gp100+

A2-/gp100+

A2-/gp100-

 
 

10-6

10-7

10-8

10-9

10-10

10-11

10-12

10-6

Mel 526

Mel 624

Mel 888

Hep 3B

Media

Patient 2 clone

7105

6606

5102

2555

187

< 10

< 10

< 10

2724

5743

< 10

< 10

< 10

Patient 5 clone

12067

11274

5323

1210

244

< 10

< 10

< 10

5114

7425

< 10

< 10

< 10

Patient 7 clone

33349

29191

26858

18575

687

220

< 10

< 10

3975

8434

< 10

< 10

< 10

  1. * Specific reactivity of cloned T cells was evaluated by tumor or peptide-specific stimulation. 1e5 cloned T cells were cocultured overnight with an equal number of melanoma tumor cells or T2 cells pulsed with either 1 μM irrelevant HIV peptide or titered concentrations of gp100:154–162 peptide, and assessed for IFN-g (pg/ml) production by standard ELISA
  2. Values 100 pg/ml and twice background are bolded and underlined.