Detection of antigen-specific IFN-γ secreting CD8+T cells by ELISpot analysis. ELISpot assay was performed as described under Methods. Number of individual gp100 and Melan A/MART-1 specific spots/105 CD8+T cells from pre and post DC/Apo-Nec vaccination samples are shown for 5/7 HLA-A*0201 patients. Data represents mean values of triplicates. Background numbers of spots were set with non – pulsed DCs and subtracted from the number of spots.