Figure 2

Patient#1's lymphocytes in vitro proliferation in response to autologous tumor Ags presented by DCs. Pre and post vaccination Patient#1 PBMC were incubated either alone, with Apo-Nec#1 cells prepared from patient#1 tumor cells or with DC/Apo-Nec#1 cells; (H3) dThd incorporation was measured as described under Methods. Results represent mean ± SD cpm (counts per minute) of triplicates. Positive controls incubated with PHA incorporated more than 7 × 104 cpm (not shown).