Figure 6

All cell types in human PBMC express functional LXRα and LXRβ. Peripheral blood mononuclear cells, monocytes, T-cells, and B-cells were purified from normal human donors (n = 3). After acclimation for 1 hour in culture, replicate cultures for each cell type from each donor were treated with either vehicle (0.1% DMSO) or LXR-623 (2 uM) for 18 hours. Cells were then harvested, RNA prepared, and qPCR was performed (in duplicate) to monitor the expression of LXRα, LXRβ, ABCA1, or ABCG1. Expression values were averaged across donor cultures for each treatment and normalized to GAPDH. A and B: mean basal levels of LXRα (A) and LXRβ (B) in vehicle-treated cultures after 18 hours in culture for each cell type. C-G: expression in vehicle treated (open bars) or LXR-623 treated cultures (black bars) of LXRα (C), LXRβ (D), ABCA1 (E, F), and ABCG1 (G). All bars represent the mean of replicate cultures from all donors +/- SEM. All fold-changes indicated in the graphs were significant with p < 0.01 by Student's t test.