Figure 4

LXR-623 treatment of human PBMC in vitro significantly increases transcription of ABCA1 and ABCG1. Peripheral blood mononuclear cells (PBMC) were purified from normal human donors (n = 3), transferred to cell culture dishes, and treated with vehicle (0.1% DMSO) or LXR-623 at either 0.05 uM or 2 uM for 16 hours. Following culture, cells were harvested and RNA was isolated for gene expression measurements of human ABCA1, ABCG1, PLTP, and GAPDH (normalizer gene) using qPCR. Bars indicate the average normalized transcript level across the three donors for each dose, +/- SEM. *p < 0.05, **p < 0.01 compared to vehicle treatment, as determined by Student's t test.