Figure 4
WB monitoring of cellular immune response to vaccination against influenza virus. Eight healthy donors were vaccinated against influenza virus. WB specimens were obtained before vaccination (day 0) and 14–112 days afterwards. WB samples (300 μl) were incubated o/n in the presence of a 0.6 μg/ml concentration of influenza hemagglutinin. Values related to the expression of IFN-γ (panel A) or IL-2 genes (panel B) were calculated by using, as reference, the expression of β-actin house keeping gene (y axes).