Figure 4
Angiogenic potential of cancer cells from monolayer and 3D-cultured CT26 cancer cells. One 3D-spheroid per mouse with a concentration of around 35,000 cells per spheroid was subcutaneously injected in 15 mice (three independent experiments; 5 mice/experiment). The same cancer cell number from monolayer-cultured CT26 was subcutaneously-injected into control mice. Subcutaneous tumors were removed on day 19th after tumor cell injection and processed for CD31 immunostaining. CD31-expression was enhanced by image analysis and CD31-expressing cell density per unit area was determined.