Figure 6

Effects of the transplanted cells on neuroprotection and recovery of impaired motor function after MCAo. A-B, Representative fluorescent photograph in non-ischemic and ischemic striatum. a, striatum; b, cortex; c, external capsule. The area where Neu-N expression was lost in the striatum in the saline-, hMNC- and hES-EC+MC-injected group represent the infarct areas (A) (mouse PECAM-1: red, Neu-N: blue. scale bar: 500 μm, ×5 magnification). B-C, Quantitative analysis of the infarct area (5 groups) in the striatum (B) and the infarct volume in the saline- and hES-EC+MC-inejcted group (C) on day 28 after MCAo.* P < 0.05. D-E, Representative fluorescent photographs on day 14 after MCAo and quantification of ss-DNA⁺ cells in the ischemic penumbral area in the saline- and hES-EC+MC-injected group. (ss-DNA: green, Neu-N: blue. Scale bar:100 μm, ×20 magnification. *P < 0.05). F, Assessment of recovery of impaired motor function by quantification of the time from the start of the exercise until collapse on an accelerating rota-rod just before the experiments (= day 0) and on day 7 and 28 after MCAo. * P < 0.05.