Figure 5
From: Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines
Western blotting for Src kinase, phospho-Src kinase py 418, FAK, phospho-FAK py 397 and py 861, immunoprecipitated (IP) EphA2, IP phospho-EphA2 and α-tubulin in (A) the panel of melanoma cell lines; and (B) HT144, Lox-IMVI, Malme-3M, Sk-Mel-5 and Sk-Mel-28 untreated (control) or treated with 100 nM dasatinib for 6 hours. (C) Western blotting for IP EphA2, IP phospho-EphA2 in Lox-IMVI untreated (control) and treated with 100 nM dasatinib for up to 48 hours.