Figure 4

Apoptotic cells (%) measured by Ann-V assay in LNCaP line grown in normal medium (white bars), charcoal-stripped (c.s.) hormone-deprived medium (grey bars) and normal medium after taxane pre-treatment (black bars). Cells were analyzed after a 1-h exposure to Doc 0.01 μM followed by a 96- or 120-h w.o. (left histogram bars), after a 96- or 120-h continuous exposure to Zol 100 μM (center histogram bars), and after exposure to the Doc-Zol combination (right histogram bars). The percentage of apoptosis in untreated cells never exceeded 10%. Error bars represent the mean standard deviation.