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Table 2 Impact of temperature on AdCCL21 transduction of DC.

From: Pre-clinical characterization of GMP grade CCL21-gene modified dendritic cells for application in a phase I trial in Non-Small Cell Lung Cancer

AdCCL21-DC

Recovery

Viability

CCL21

37°C

RT

37°C

RT

37°C

37°C

    

ng/1 × 106

ng/1 × 106

ng/1 × 106

Ng/1 × 106

%

24 h

48

24 h

48

50.7 ± 14

59 ± 0.03

75 ± 0.2

*93.6 ± 0.02

1.1 ± 0.7

1.9 ± 1.3

2.9 ± 2.1

3.7 ± 2.5

  1. • p = 0.05
  2. In preliminary experiments (n = 4), the impact of temperature on adenoviral transduction of human DC was evaluated. Cell recovery, viability by trypan blue exclusion, and CCL21 production are shown at 37°C and RT.
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Journal of Translational Medicine

ISSN: 1479-5876

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