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Table 2 Impact of temperature on AdCCL21 transduction of DC.

From: Pre-clinical characterization of GMP grade CCL21-gene modified dendritic cells for application in a phase I trial in Non-Small Cell Lung Cancer

AdCCL21-DC
Recovery Viability CCL21
37°C RT 37°C RT 37°C 37°C
     ng/1 × 106 ng/1 × 106 ng/1 × 106 Ng/1 × 106
% 24 h 48 24 h 48
50.7 ± 14 59 ± 0.03 75 ± 0.2 *93.6 ± 0.02 1.1 ± 0.7 1.9 ± 1.3 2.9 ± 2.1 3.7 ± 2.5
  1. • p = 0.05
  2. In preliminary experiments (n = 4), the impact of temperature on adenoviral transduction of human DC was evaluated. Cell recovery, viability by trypan blue exclusion, and CCL21 production are shown at 37°C and RT.