| Â | MNC Yield (BC) | MNC Recovery (AC) | MNC Viability (AC) | DC Yield (BT) | DC Viability* (BT) | AdCCL21 Recovery (AT) | AdCCL21 Viability (AT) |
|---|
N = 17 | 1 × 109 | 1 × 109 | % | 1 × 106 | % | % | % |
|  | 1.0 ± 0.7 | 0.66 ± 0.4 | 92.9 ± 4.3 | 21.3 ± 11 | 95.8 ± 1.7 | 65.9 ± 7.4 | 88 ± 13% |
- * N = 16
- DC were generated from cryopreserved MNC obtained from leukapheresis (LK) samples under standard culture conditions (6 days culture of adherent MNC in GM-CSF and IL-4), as described in Materials and Methods. A summary of the yield, recovery, and viability of MNC is reported before cryopreservation (BC), after cryopreservation (AC), before adenoviral transduction (BT), and after adenoviral transduction (AT) with AdCCL21 at room temperature. The values express the average ± SD of at least 16 independent experiments.
