Figure 5
CCL21-DC secrete immunostimulatory cytokines. Human monocyte-derived DC were transduced with culture medium, AdCCL21 (1167 VP/cell), or AdCV by the centrifugation method. 2.0 × 105 DC, CCL21-DC, or CV-DC were seeded into a 48 well plate in 1 ml of culture medium in the presence or absence of the indicated stimuli. For IL-12 assays, cells were stimulated with IFN-γ + LPS, and for IP-10 and MIG assays cells were stimulated with LPS only. The supernatants from stimulated and unstimulated DC were harvested after 48 h culture. IL-12p70 (A), IP-10 (B), and MIG (C) were measured by ELISA. Values refer to cytokine concentration expressed in ng/million cells. One representative experiment of four is shown.