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Figure 3 | Journal of Translational Medicine

Figure 3

From: Relative quantification of TCR Vbeta-chain families by real time PCR for identification of clonal T-cell populations

Figure 3

Serial dilutions of CCRF-CEM (Vβ9) and MOLT-16 (Vβ2, Vβ18) cell lines in PBMCs of a healthy volunteer. Measured percentages of all families in case of 50, 500, 5000, 50 000 CCRF-CEM cells (A) or MOLT-16 cells (B) added to 100 000 PBMCs of volunteer 2. Dilutions, RNA extraction, cDNA synthesis and qRT PCR are performed in duplicated. Mean percentages of each dilution step were shown. P j : -chain relative expression.

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