Figure 2From: Relative quantification of TCR Vbeta-chain families by real time PCR for identification of clonal T-cell populationsSerial dilutions of JURKAT cells expressing Vβ-family 8 spiked in PBMCs of three healthy volunteers. (A) measured percentages of family 8 in case of 50, 500, 5000, 50 000 JURKAT cells added to 100 000 PBMCs; (B) mean percentages of the three volunteers compared with theoretical values calculated assuming 5.88% family 8 without JURKAT cells and 70% TCR α/β positive cells in 100 000 PBMCs. P j : Vβ-chain relative expression.Back to article page