Cytomegalovirus-specific T-cell responses and viral replication in kidney transplant recipients

Table 2 Percentage of CMV-antigen specific interferon gamma (IFN-γ) producing CD4+ and CD8+ T-cells in healthy donors and kidney transplant patients.

		Kidney transplant (KT) patients				Healthy donors (HD)
			CMV D+R+/D-R+ n = 48, m = 79	CMV D+R- n = 25, m = 50	p-values*	CMV seropositive n = 13, m = 19	CMV seronegative n = 17, m = 17	p-values*
CMV-	CD3⁺ CD4⁺	median (range)	0.05 (0.00–4.35)	0.01 (0.00–0.14)	< 0.001	1.03 (0.03–6.19)	0.01 (0.00–0.22)	< 0.001
lysate	CD3⁺ CD8⁺	median (range)	0.02 (0.00–1.31)	0.00 (0.00–0.07)	0.003	0.49 (0.00–4.57)	0.00 (0.00–0.56)	< 0.001
pp72	CD3⁺ CD4⁺	median (range)	0.04 (0.00–0.36)	0.02 (0.00–0.26)	0.027	0.08 (0.01–2.83)	0.00 (0.00–0.03)	0.001
peptides	CD3⁺ CD8⁺	median (range)	0.05 (0.00–0.68)	0.03 (0.00–0.34)	0.056	0.2 (0.00–3.47)	0.00 (0.00–0.03)	0.002
pp65	CD3⁺ CD4⁺	median (range)	0.02 (0.00–0.50)	0.01 (0.00–0.55)	0.013	0.11 (0.00–4.70)	0.00 (0.00–0.02)	0.018
peptides	CD3⁺ CD8⁺	median (range)	0.02 (0.00–0.62)	0.00 (0.00–0.25)	0.001	0.21 (0.00–2.80)	0.00 (0.00–0.20)	0.009

Note. The results are given as frequencies of CD3⁺ CD4+ or CD3+CD8+ lymphocytes as detailed in Materials&Methods. The number of measurements exceeds the number of patients because some patients were tested at more than one visit. CMV, Cytomegalovirus; HD, healthy donors; KT, kidney transplant; n, number of patients; m, number of independent blood sampling and measurements. CMV seronegative KT patients had a D(+)R(-) constellation. CMV seropositive KT patients included both D(+)R(+) and D(-)R(+) constellations. (*) Mann-Whitney U test.

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