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Figure 6 | Journal of Translational Medicine

Figure 6

From: Whole tumor antigen vaccination using dendritic cells: Comparison of RNA electroporation and pulsing with UV-irradiated tumor cells

Figure 6

Antitumor efficacy of RNA electroporated DCs. (A) Growth of flank TC-1 tumors in animals previously vaccinated with DCs electroporated with TC-1 cell RNA, UV-irradiated TC-1 cells, mock DCs or non-treated. Animals were challenged by injecting 2 × 104 tumor cells. (n = 5 each group). Data are representative of three independent experiments with similar results. Day 30: RNA vs. UVB: p < 0.01; RNA vs. PBS: p < 0.01; RNA vs mock: p < 0.01; UVB vs. PBS: p < 0.01; UVB vs. mock: p < 0.01; PBS vs. mock: non significant. Mann-Whitney test. (B) CD3 and CD8 staining of tumors obtained from animals vaccinated with DCs electroporated with TC-1 cell RNA (RNA), DCs pulsed with UVB-irradiated TC-1 cells (UVB), or mock electroporated DCs. Magnification: 200X. (C) Counting of CD3 cells infiltrating TC-1 tumors. Experimental groups as in A. RNA vs. UVB: p < 0.001; RNA vs. mock: p < 0.001; UVB vs. mock: p < 0.001. Kruskal-Wallis non parametric ANOVA test. Dunn's multiple comparisons test. (D) Percentage of CD8+ among CD3+ cells infiltrating TC-1 tumors. Experimental groups as in A. RNA vs. UVB: p < 0.05; RNA vs. mock: p < 0.001; UVB vs. mock: NS (Kruskal-Wallis non parametric ANOVA test. Dunn's multiple comparisons test).

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