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Figure 4 | Journal of Translational Medicine

Figure 4

From: Whole tumor antigen vaccination using dendritic cells: Comparison of RNA electroporation and pulsing with UV-irradiated tumor cells

Figure 4

Expression of HPV-16 E6 and E7 antigens in retrovirus-transduced ID8 cells. (A) RT-PCR analysis for HPV-16 E6 and E7 transcripts in ID8 cells transduced with HPV-16 E6 and E7 genes; ID8 cells transduced with empty vector (negative control); and TC-1 cells (positive control). M: molecular markers. (B) Real-time quantitative PCR analysis of E6 and E7 transcripts in ID8 cells transduced with HPV-16 E6 and E7 genes and ID8 cells carrying empty vector. (C) Western blot analysis of cell lysate showing presence of E6 protein in samples obtained from ID8 E6/7 cells but not in ID8 cells carrying empty vector. M: molecular markers. (D) Flow cytometry analysis of intracellular E6 and E7 proteins in ID8 cells transduced with HPV-16 E6 and E7 genes. (grey): ID8 E6/7 cells; (white): ID8 cells transduced with empty vector, (dotted line): isotype control. (E) Expression of MHC-I by ID8-E6/7 cells. (grey): ID8 E6/7 cells; (white): isotype control.

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