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Figure 3 | Journal of Translational Medicine

Figure 3

From: Whole tumor antigen vaccination using dendritic cells: Comparison of RNA electroporation and pulsing with UV-irradiated tumor cells

Figure 3

(A) Expression of maturation markers in E7+CD11c+ gated cells 72 hours after electroporation with TC-1 RNA (50 μg/106 cells). LPS and TNF-α were added to the culture medium 24 hours after electroporation. (white): specific antibody; (grey): isotype control. The experiment was repeated two times with similar results. (B) Expression of markers in CD11c+ gated cells 72 hours after phagocytosis of UVB-irradiated TC-1 cells. LPS and TNF-α were added to the culture medium after 24 hours of coincubation. (white): specific antibody; (grey): isotype control. (C) Proliferation of allogeneic BALB/c splenocytes induced by C57BL/6 DCs electroporated with TC-1 cell RNA (RNA) or pulsed with UV-irradiated TC-1 cells (UVB). DCs were treated with TNF-α and LPS. Data were obtained 48 hours after stimulation of splenocytes and are expressed as the mean ± SEM of two experiments with triplicate observations per experiment. (D) Migration of DCs towards MIP-3α or MIP-3β 72 hours after electroporation with TC-1 RNA (RNA) or pulsing with UV-irradiated TC-1 cells (UVB). LPS and TNF-α were added to the culture medium after 24 hours of coincubation. Chemotactic index is defined as the fold increase in cell migration caused by the chemotactic factors. Data are expressed as the mean ± SD of two experiments with quadruplicate observations per experiment.

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