Figure 1

Gene expression profile: comparison between antigen specific and convivial non specific CD8+ T cells. A) representative example of in vitro expansion of Flu M1:58-66-specific CD8 T cells in the presence of 300 IU/IL-2 and purity of the sorting procedure by high speed flow cytometry (FACSVantage SE); B) multiple dimensional scaling representing the four populations of CD8 T cells based on the complete data set of 16,726 cDNA clones. CD8 expressing, CSFE^high, Flu/tHLA negative T cells (non Flu specific) are referred to as IL-2 while CD8 expressing, CSFE^low, Flu/tHLA positive T cells are referred to as IL-2+Flu; C) Venn diagram displaying the extent of overlap between genes differentially expressed by Flu-specific and non Flu-specific CTLs from the same culture compared to quiescent CD8-expressing T cells; D) Multiple dimensional scaling plot representing Flu-specific and convivial CD8 T cells distribution using the complete data set of 16,726 cDNA clones.