3A. i. The PCR result shows 12/19 positive cases for PTTG-1 at the transcriptional level. ii. Representative cases of patient MM plasma cells. In the (P) panel, the cytoplasmic staining is shown by ICC, IF and FACS. Also the NP panel shows a positive result for surface staining by ICC, IF and FACS methods. 3B. i. The investigation of the 6 established MM cell lines gave positive band signals in the four cases of KMS-11, 8226, ARK-B and ARP-1. ii. The evaluation of PTTG-1 protein expression showed only cytoplasmic but not surface positive reaction. The case of the permeabilized (P) and not permeabilized (NP) 8226 cell line is shown by means of ICC, IF and FACS.