Figure 1

Diagram of a cell-based genomic-screening for anti-proliferative components of LASH. Tumor cells were stably transfected either with a siRNA array or a ribozyme library. After exposure to the crude extracts, gene targets were identified. Bioassay-guided purification was done to isolate the active component for the crude extracts. The p53 gene, a convenient target, was among the initial genes identified with LASH.