Hypermethylated MAL gene – a silent marker of early colon tumorigenesis

Table 2 PCR primers used for MSP and bisulphite sequencing.

Primer set	Sense primer	Antisense primer	Frg. Size, bp	An. Temp	Fragment location*
MAL MSP-M	TTCGGGTTTTTTTGTTTTTAATTC	GAAAACCATAACGACGTACTAACGT	139	56	-71 to 68
MAL MSP-U	TTTTGGGTTTTTTTGTTTTTAATTT	ACAAAAACCATAACAACATACTAACATC	142	56	-72 to 70
MAL BS_A	GGGTTTTTTTGTTTTTAATT	ACCAAAAACCACTCACAAACTC	236	53	-68 to 168
MAL BS_B	GGAAAAATGAAGGAGATTTAAATTT	AATAACCTAAACRCCCCC	404	50	-427 to -23

Abbreviations: MSP, methylation-specific polymerase chain reaction; BS, bisulphite sequencing; M, methylated-specific primers; U, unmethylated-specific primers; Frg. Size, fragment size; An. Temp, annealing temperature (in degrees celsius). *Fragment location lists the start and end point (in base pairs) of each fragment relative to the transcription start point provided by NCBI (RefSeq ID NM_002371).

ISSN: 1479-5876