Figure 5
From: Transient T cell depletion causes regression of melanoma metastases
Flow cytometric scatter plots demonstrating the de novo appearance of MART1-, gp100- and tyrosinase-specific CD8+ T cells after one cycle of DAB/IL2. Whole blood was collected from patient P16 during cycle one of DAB/IL2 administration just prior to (day 0) and 21 days after the first dose of DAB/IL2. The peripheral blood mononuclear cells were isolated from the whole blood by Ficoll gradient centrifugation and stained with a PE-labeled anti-CD8 monoclonal antibody and the indicated APC-labeled tetrameric HLA-A2*0201/peptide conjugates.