Differential susceptibility of C57BL/6NCr and B6.Cg-Ptprca mice to commensal bacteria after whole body irradiation in translational bone marrow transplant studies

Table 1 C57BL/6NCr are sensitive to irradiation doses >1000 cGy.

Inoculum type	Recipients	First day Symptoms observed post BMT	#affected	% affected	Treatment	Rads (cGy)	Irradiator
BM+ Tc	B6 (Ly5.1)	6	2/4	50		1300 split	Xray
BM + splenocytes	B6 (Ly5.1)	7	2/4	50		1100 single	Xray
BM + Tc	B6 (Ly5.1)	9	2/4	50		1100 single	Xray
BM+Tc	B6 (Ly5.1)	7	1/3	33	EL-4	1100 single	Xray
BM+Tc	B6 (Ly5.1)	7	3/12	25	EL-4	1100 single	Xray
BM+T c	B6 (Ly5.1)	6	2/6	33	EL-4	1100 single	Xray
BM+Tc	B6 (Ly5.1)	6	8/8	100	EL-4	1100 single	Xray
BM+Tc	B6 (Ly5.1)	6	11/20	55		1100 single	gamma
BM+Tc	B6 (Ly5.1)	6	2/4	50		1100 single	gamma
BM+Tc	B6 (Ly5.1)	6	4/5	80		1100 split	gamma
BM+Tc	B6 (Ly5.1)	5	4/6	66		1300 split	gamma
BM+Tc	B6 (Ly5.1)	5	9/11	81	MBL-2	1300 split	gamma
BM+DC	B6 (Ly5.1)	4	5/5	100	DC	1100 single	X-ray
BM+Tc	B6 (Ly5.1)	5	5/5	100		1100 single	gamma
BM+Tc	B6 (Ly5.1)	5	3/9	33		1000 single	gamma

C57BL/6NCr underwent BMT and exhibited unexpected deaths. Animals were reconstituted with bone marrow (BM) and T-cells (Tc), or bulk splenocytes (splenocytes) or dendritic cells (DC). Symptoms started as early as day +4 post-transplantation. Some animals received MBL-2 or EL-4 leukemia cells. Ionizing radiation was given in a single or fractionated (split) dose or administered with an X-ray diffraction instrument. Split regimens were delivered in two equal amounts separated at least 3 hours apart.

ISSN: 1479-5876