A majority of EphA2
-reactive CD8+ cells in AA-1-derived PBMC are central memory cells. Freshly thawed PBMCs were stained with anti-CD8 mAb, EphA2883–891-tetramer, anti-CD45RA mAb, and anti-CCR7 mAb and analyzed as described in Materials and Methods. Black, gray and white columns indicate the percentage of each population in EphA2883–891-specific CD8+ cells derived from AA-1, AO-1, and healthy donor, respectively. Bars indicate standard errors. Higher percentages of central memory T cells (CD45RA-/CCR7+) and effector memory T cells (CD45RA-/CCR7-) were observed in EphA2-tetramer+/CD8+ T cells of AA-1 compared to those of AO-1 and the healthy donor (P < 0.05; ANOVA).