Type-1 polarized DCs are efficient producers of IL-12 p70. In UPCI 99-111, on each vaccination day, a small aliquot of DCs (8 × 104) were tested for IL-12 production capability in comparison to DCs that were not stimulated with the type-1 cytokine-mixture (standard DCs). This was done with 24 hr stimulation of DCs (20 × 103per well, duplicates) with CD40L-transfected J558 cells (50 × 103per well). Supernatant was harvested and the production of IL-12 p70 was assayed by specific ELISA. Values indicate averages of duplicate samples. Bars indicate standard errors.