Figure 4

Analysis of PSC supernatants and cell lysates following coculture with PBMC. (A) PSC cell culture supernatants were subjected to ELISA following coculture with donor, CP and sepsis PBMC. Hatched bars: PSC supernatants. White bars: Pooled donor, CP or sepsis PBMC and corresponding PSC supernatants. Black bars: coculture supernatants. *p < 0.05 as assessed using the Mann-Whitney U test. (B) Immunoblot analysis of fibronectin in PSC cell lysates. Densitometry shown as percent of control adjusted to the corresponding gamma-tubulin density.