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Figure 4 | Journal of Translational Medicine

Figure 4

From: A function blocking anti-mouse integrin α5β1 antibody inhibits angiogenesis and impedes tumor growth in vivo

Figure 4

Annexin V cell death assays. Primary mouse endothelial cells were incubated with 339.1, 517-2 or 13E9-20 (10 μg/ml) for 16 hours and assessed for Annexin V-Alexa Fluor 488 binding by flow cytometry. Cells were counterstained with propidium iodide (PI) to follow non-specific death and the percentage of cells that stained positive for Annexin V and negative for PI was plotted. Representative results from three individual experiments are shown, (A). Only antibody 339.1, which does not cross-react with human α5β1, elicited cell death in these cells. A dose response curve for 339.1 in this assay using SVR murine angiosarcoma cells revealed an EC50 of 5.3 nM, (A).

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