Figure 8
Effect of pretreatment with Phenoxodiol on the apoptotic cascade. YUMAC cells received either no treatment, 200 μg/mL of Carboplatin for 24 hours, 10 μg/mL of Phenoxodiol for 2 hours, or 10 μg/mL of Phenoxodiol for 2 hours followed by 200 μg/mL Carboplatin for 24 hours. Both pro- and antiapoptotic protein expression was determined by Western blot analysis. Activity of caspase-3 was determined using the Caspase-Glo 3\7 assay.