Figure 1

NMT activity in peripheral blood mononuclear cells (PBMC) and Bone Marrow Cells (BMC) of normal and colorectal tumor bearing rats a; Isolated peripheral blood mononuclear cells from peripheral blood of control or tumor bearing rat were assessed for NMT activity. NMT activity was assayed using cAMP-dependent protein kinase derived peptide substrate. Values are mean ± SD of three independent experiments. Inset: Western blot analysis of peripheral blood mononuclear cells and bone marrow cells of normal and colorectal tumor bearing rats. Proteins (25 μg) from PBMC or BMC of control or tumor bearing rats were subjected to 10% SDS-PAGE, transblotted onto nitrocellulose membrane and were probed with monoclonal anti-human NMT antibody (1:250 dilutions).