Figure 8
NO release of thioglycolate-elicited macrophages. Thioglycolate-elicited peritoneal macrophages and effector T cells (TE) were plated into a 24-well plate and co-cultured without or with D5, MPR-4 (a syngeneic but unrelated tumor), or anti-CD3. Macrophages and tumor cell co-cultures without the addition of effector T cells was an additional control. Supernatants were harvested after 4 hours and the release of NO determined in duplicate by GRIES reagent using commercially available reagents. Data are presented as the mean of three independent experiments (SEM) * p < 0.05