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Figure 7 | Journal of Translational Medicine

Figure 7

From: Therapeutic T cells induce tumor-directed chemotaxis of innate immune cells through tumor-specific secretion of chemokines and stimulation of B16BL6 melanoma to secrete chemokines

Figure 7

Chemotaxis-assay of DJ2PM macrophages either pretreated with (black columns) or without (white bars) PTx (0.5 g/ml). Migration towards serum control, MIP-1α/MIP-1β, effectotr T cells (TE), D5 or D5 co-incubated with TE was determined after 4 hours. MIP-1α/MIP-1β, TE, D5 and TE co-cultured with D5 were plated into the bottom chamber of a 24 well transwell plate for 12 hours. Subsequently 3.5 × 105 peritoneal macrophages (DJ2PM) were added to the top chamber for 4 hours. The number of macrophages that migrated into the lower chamber was determined by flow cytometry as the percentage of CD11b+/CDFA-SE+ cells. Data are presented as the mean ± SE of three independent experiments (* p < 0.05).

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