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Figure 4 | Journal of Translational Medicine

Figure 4

From: Therapeutic T cells induce tumor-directed chemotaxis of innate immune cells through tumor-specific secretion of chemokines and stimulation of B16BL6 melanoma to secrete chemokines

Figure 4

a) mRNA-expression of KC, MCP-1, IP-10, RANTES and MIG in unstimulated and stimulated D5 tumor cells. D5 tumor cells were cultured alone or stimulated with IFN-γ (2, 10, 20 ng/ml) or TNF-α (3.5, 35, 350 U/ml) for 2 and 12 hours. Tumor cells were harvested and mRNA expression determined by RT-PCR. b) Chemokine mRNA-expression of MIP-1α and MIP-1β in unstimulated and stimulated D5 tumor cells. D5 tumor cells were cultured alone or stimulated with IFN-γ (20 ng/ml) and TNF-α (350 U/ml) for 2 and 12 hours. D5 tumor cells were harvested and mRNA expression determined by RT-PCR. RNA isolated from the macrophage cell line J774 was stimulated with IFNγ (20 ng/ml) and LPS (10 μg/ml) for 4 h and used as a positive control. Negative controls included water instead of cDNA.

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