MIP-1α MIP-1β RANTES and IP10 mRNA-expression by effector T cells. Effector T cells were cultured alone (unstimulated control), with D5 (specific tumor stimulation), with a syngeneic but unrelated tumor MCA-310 (specificity control), or with anti-CD3. T cells were harvested 6 h later to determine MIP-1α MIP-β, RANTES and IP10 mRNA expression. RNA isolated from the macrophage cell line J774 was stimulated with IFNγ (20 ng/ml) and LPS (10 μg/ml) for 4 h and used as a positive control for chemokine message. Negative controls included water instead of cDNA. Hypoxanthine phosphoribosyltransferase 1 (HPRT) is a commonly used reference gene that was used as a control.