Figure 4

osteogenic and adipogenic differentiation of adherent cells from SVF culture. A: osteogenic differentiation (Alizarin Red staining); B: control; C: adipogenic differentiation (Oil O Red staining); D: control (100×). E: gene expression profile of un-induced cells compared with cultured ASC following osteogenic and adipogenic induction. GAPDH: glyceraldehide phosphate dehydrogenase; OSC: osteocalcin; OSP: osteopontin; PPARγ: peroxisome proliferator-activated receptor γ; LPL: lipoprotein lipase; FABP4: fatty acid binding protein 4.