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Table 1 Colony formation by day 0 mononuclear cells and by day 14 expanded CD34+ cells.

From: Optimal ex vivo expansion of neutrophils from PBSC CD34+ cells by a combination of SCF, Flt3-L and G-CSF and its inhibition by further addition of TPO

  % BFU-E % CFU-GM + % CFU-G BFU-E per thousand cells CFU-GM per thousand cells % CD34 in CFC assay CD34 fold expansion in culture
Day 0       
exp 28 74 26 35.9 12.4 2.6  
exp 29 74 26 8.6 3.0 1.2  
exp 32 72 28 5.0 1.9 0.4  
Day 14       
exp 28 SF 10 90 3.0 28.5 100 17.5
exp 28 SFG 5 95 0.8 16.1 100 33.4
exp 29 SF 18 82 1.1 4.9 100 1.8
exp 29 SFG 9 91 0.4 4.5 100 2.8
exp 32 SF 13 87 0.67 4.7 100 10.8
exp 32 SFG 0 100 0 4.7 100 32.4
  1. CFC comparisons in 3 sets of mobilised PBSC before culture (day 0: mononuclear cell isolates, not CD34+ enriched) and after culture (day 14: expanded CD34+ enriched cells) with SCF/Flt3-L (SF) and with SCF/Flt3-L + G-CSF (SFG). The proportional (percent) results were used for qualitative comparisons. Some indication of quantitative responses can be obtained from CFC numbers, which may relate to CD34+ numbers allowing for CD34+ expansion.