| |
% BFU-E
|
% CFU-GM + % CFU-G
|
BFU-E per thousand cells
|
CFU-GM per thousand cells
|
% CD34 in CFC assay
|
CD34 fold expansion in culture
|
|---|
|
Day 0
| | | | | | |
|
exp 28
|
74
|
26
|
35.9
|
12.4
|
2.6
| |
|
exp 29
|
74
|
26
|
8.6
|
3.0
|
1.2
| |
|
exp 32
|
72
|
28
|
5.0
|
1.9
|
0.4
| |
|
Day 14
| | | | | | |
|
exp 28 SF
|
10
|
90
|
3.0
|
28.5
|
100
|
17.5
|
|
exp 28 SFG
|
5
|
95
|
0.8
|
16.1
|
100
|
33.4
|
|
exp 29 SF
|
18
|
82
|
1.1
|
4.9
|
100
|
1.8
|
|
exp 29 SFG
|
9
|
91
|
0.4
|
4.5
|
100
|
2.8
|
|
exp 32 SF
|
13
|
87
|
0.67
|
4.7
|
100
|
10.8
|
|
exp 32 SFG
|
0
|
100
|
0
|
4.7
|
100
|
32.4
|
- CFC comparisons in 3 sets of mobilised PBSC before culture (day 0: mononuclear cell isolates, not CD34+ enriched) and after culture (day 14: expanded CD34+ enriched cells) with SCF/Flt3-L (SF) and with SCF/Flt3-L + G-CSF (SFG). The proportional (percent) results were used for qualitative comparisons. Some indication of quantitative responses can be obtained from CFC numbers, which may relate to CD34+ numbers allowing for CD34+ expansion.
