Skip to main content
Figure 2 | Journal of Translational Medicine

Figure 2

From: Optimal ex vivo expansion of neutrophils from PBSC CD34+ cells by a combination of SCF, Flt3-L and G-CSF and its inhibition by further addition of TPO

Figure 2

Change in CD34 expression of cultured cells. Anti-CD34-PE staining of 14-day cultured cells stimulated with 100 ng/ml SCF + 10 ng/ml Flt3-L with or without 100 ng/ml G-CSF. (a) SCF + Flt3-L alone; (b) SCF + Flt3-L with G-CSF (solid line = day 0; dotted line = day 7); (c) no anti-CD34, isotype control (for SCF + Flt3-L alone, but controls for the other cultures show overlapping peaks). Gated on intact cells (forward/side scatter) and CD45 staining.

Back to article page