Figure 5

Antigen-specific T helper response is amplified when vaccine-treated DCs are further modulated with R-848 and Poly I:C. T cells were processed as described in the legend to Fig. 3. Briefly, responder T cells from six cancer patients and autologous DCs were co-cultured at a ratio of 10:1 for 5 days in sextuplicates. Cultures were pulsed with BrdU reagent during the last 16 hours after which DNA synthesis was measured by assessing BrdU uptake with a BrdU ELISA kit.