Figure 4

Cytolytic T cell response to vaccine in patient PBMCs includes a high affinity T cell compartment with specificity for two HLA-A2-restricted hCGβ-derived epitopes. Granzyme B-ELISpot determination of antigen specific T cells in three cancer patients shows significant effector function is mediated by CD8+ T cells compared to NK cell activity (K562 lysis). T cells were assessed for activity in triplicates using peptide-loaded TAP -/- T2 cells in the presence of 3 μg/ml β 2 m in AIM-V medium for 3 hours at 37°C before transfer to GrB ELISpot plates for a further 16 hour incubation and processed as described under Methods.