Molecular purging of multiple myeloma cells by ex-vivo culture and retroviral transduction of mobilized-blood CD34+ cells

Table 1 Results of CD34⁺ cell selection, culture and transduction

Patient	Number of CD34⁺ selected cells (× 10³)	Purity of CD34⁺ selected cells (%)	Transduction (%)	Number of ΔLNGFR selected cells (× 10³)	Purity of ΔLNGFR selected cells (%)	%CD34⁺ cells among ΔLNGFR selected cells	CD34⁺ transduced cells vs initial CD34⁺ cells (ratio)
MM 1	1082.0	78.6	29.8	156.2	92.8	72.4	13.3
MM 2	1913.0	33.6	20.9	36.0	85.5	43.6	24.4
MM 3	887.0	87.3	32.4	456.0	93.2	60.2	35.4
MM 4	1239.0	91.2	26.7	394.6	96.9	45.1	15.8
MM 5	1229.0	95.8	20.1	238.6	85.4	62.4	12.7
MM 6	1576.0	80.6	32.1	1520.0	94.5	62.2	74.4
MM 7	4285.0	37.1	31.4	729.0	90.4	67.6	31.0
MM 8	341.0	96.8	8.5	96.4	91.7	79.7	23.3
MM 9	1000.0	86.1	38.5	490.0	89.3	68.5	39.0
MM 10*	1000.0	97.2	56.1	266.0	89.3	78.2	21.4
MM 11	500.0	77.6	33.7	78.0	77.9	75.0	15.1
MM 12	15000.0	97.2	17.0	3900.0	88.0	76.3	20.4
MM 13	1000.0	50.4	26.0	175.0	95.0	71.0	24.7
MM 14*	22300.0	98.0	56.2	7990.0	97.8	67.5	24.1
MM 15	4400.0	76.0	18.0	576.0	93.3	79.0	13.8
MM 16	2890.0	79.0	20.0	396.0	97.3	84.0	14.6
MM 17	900.0	95.0	28.5	148.0	97.4	66.0	11.4
MM 18	1740.0	78.4	26.0	234.0	95.4	38.4	6.6
MM 19	2890.0	78.0	30.0	560.0	99.8	67.9	16.9
MM 20	2100.0	98.7	26.5	170.0	98.8	89.8	7.4
Mean ± SD		80.6 ± 19%	28.9 ± 12%		92.5 ± 5%	67.7 ± 13%	22.3 ± 15%

CD34⁺ enriched cells of 19 patients were transduced with the ΔLNGFR retroviral vector, selected for transgene after 48 hours, and analyzed by flow cytometry. The last column shows the ratio between the number of CD34⁺ cells in the ΔNGFR⁺ selected cell population, and the initial number of CD34⁺ cells. * cells derived from the same patient.

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