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Figure 4 | Journal of Translational Medicine

Figure 4

From: Differential in vitro inhibitory activity against HIV-1 of alpha-(1-3)- and alpha-(1-6)-D-mannose specific plant lectins : Implication for microbicide development

Figure 4

(A). Lack of toxicity of HHA and GNA towards dendritic cells (MDDC). MDDC were cultured with concentrations of products for 24 h. After washing, culture viability was determined by using the MTT cytotoxicity assay. The values given are the percentage of viability. (B). Interactions of plant lectins with surface receptors of dendritic cells. Dendritic cells (MDDC) were incubated with or without each labelled-lectin at 4°C. Cells were then analyzed by confocal microscopy. (C and D). Ability of lectins to inhibit uptake of HIV-1Ba-L cell-free particles on dendritic cells. MDDC were co-incubated with non-toxic concentrations of HHA (C), or GNA (D) or mannan (100 μg/ml) and 1 ng p24 antigen of HIV-1Ba-L were added for 1 h. The quantity of attached-virus was evaluated by measurement of p24 antigen by ELISA. Means of three independent experiments are presented ; error bars represent standard deviations. *< 0.05

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