Figure 3

Ability of lectins to inhibit transcytosis of HIV-1_J-RCSF free particles through a tight monolayer of endometrial epithelial cell (HEC-1A). HIV-1_J-RCSF (5 ng p24 antigen) was co-incubated with a non-toxic concentrations of each lectins or mannan (100 μg/ml) on the HEC-1A apical pole cultured in dual-chamber by the transwell system for 1 h. Results of an experiment performed in duplicate are expressed as the quantity of virus recovered in the basal chamber in the presence of HHA (A) and GNA (B), or in the absence of lectins. Means of three independent experiments are presented ; error bars represent standard deviations. *< 0.05, **< 0.01.