Figure 4

Spleens from mice immunized against a mixture of TRP2:180–188 and modified p53:232–240 in VM contained high numbers of TRP2:180–188-specific (right bar) and modified p53:232–240-specific (left bar) IFN-γ producing cells. In contrast, spleens from mice immunized against a mixture of TRP2:180–188 and modified p53:232–240 without VM contained high numbers of TRP2:180–188-specific IFN-γ producing cells but low numbers of modified p53:232–240-specific IFN-γ producing cells. The spleens of mice immunized with VM-formulated vaccine without CpG ODN had low numbers of both TRP2:180–188- and modified p53:232–240-specific IFN-γ producing cells as did the spleens of mice from mice immunized without VM.