Figure 3

Ex-vivo detection of modified p53:232–240-specific IFN-γ producing splenocytes (spot forming cells, SFC) in mice 8 days following a single immunization against modified p53:232–240 in VM. Mice immunized against modified p53:232–240 peptide in VM with CpG and PADRE adjuvants produced the largest number of cytotoxic T cells. Without VM or omission of CpG from the VM formulation produced low numbers of modified p53:232–240-specific IFN-γ producing splenocytes. Vaccination against an irrelevant peptide (IrrPep) also produced low numbers of IFN-γ producing splenocytes.