Figure 7

Measurement of intracytoplasmatic IL-10 and IL-12 in DC/Apo-Nec cells. In a four color experiment, intracytoplasmatic IL-10 and IL-12 cytokines were measured by FACS in iDCs (A) or DC/Apo-Nec (B) as described under Methods. Cells were labeled red (iDCs) and green (Apo-Nec cells) and after phagocytosis only the double-labeled population (DC/Apo-Nec cells) was analyzed with APC-anti IL-10 and PerCP IL-12 Abs after 8 hs treatment with Brefeldin A. 10,000 events per point were analyzed.