CCR7 expression and in vitro DCs migration in response to MIP-1α and MIP-3β. CCR7 expression (left panel) was analyzed by FACS in iDCs (A), LPS-treated DCs (B) and DC/Apo-Nec (C). Grey histograms represent the corresponding isotype matched control Ab. In each histogram the percentage of positive cells and the Mean are indicated. In vitro migration in response to MIP-1α and MIP-3β was evaluated (right panel) for iDC, LPS-treated DCs and DC/Apo-Nec cells as indicated. A control for random migration was done using RPMI medium. The number of migrating cells was calculated as the mean ± SD of cells counts in five medium-power (40×) fields/well performed in triplicate. A representative experiment of three is shown *p < 0.05 (Student's t-Test).