Figure 4

Maturation of DCs after Apo-Nec cells phagocytosis. A – DCs phenotype was studied before (iDCs) and after coculture with Apo-Nec cells (DC/Apo-Nec) analyzing CD14, CD11c and CD1a expression as described under Methods. In each case isotype-matched controls were performed. B – DCs marker expression of iDCs, LPS-treated DCs and DC/Apo-Nec. Cells were stained with FITC-labeled monoclonal antibodies as described under Methods and evaluated by FACS. Grey histograms represent the corresponding isotype matched control Abs. A representative experiment of six is shown. In each histogram the percentage of positive cells and the mean fluorescence intensity (Mean) are indicated. C – Endocytic activity of iDC, control LPS-treated DCs and DC/Apo-Nec were evaluated by the uptake of FITC-Dx as described under Methods. Percentage of endocytic DCs and Mean are indicated. A representative experiment of four is shown.